ADENYLYL CYCLASE

Richard Crook and JR Polansky (Inv. Ophth. & Vis. Sci 1994;35< 3374) showed that forskolin, a known activator of
adenylyl cyclase (AC), could increase rubidium uptake in fetal nonpigmented epithelium (NPE) from the ocular ciliary body.
Here are results from my experiments in the companion pigmented epithelia (CPE or PE) that verify that AC is involved in
regulation of the NaK2Cl cotransporter in the CPE cell line. Since the experiments were already being published for the NPE cells, I merely tested my technique in CPE.

Note that upon the addition of bumetanide (red squares), there is
significantly less uptake of rubidium when compared to a trial with
no bumetanide added (black circles). This implies that function of
the NaK2Cl cotransporter is essential to this process. This may
mean that the cotransporter is directly effected, for example by
phosphorylation events, as is shown in the diagram, or alternatively,
transcellular resistance may be decreased by the opening of ion channels,
such as the chloride channel depicted as being phosphorylated by a
PKA catalytic subunit at the other end of the cell.

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